Endocrine Abstracts

The aim of por in-vitro studies was to examine the involvement of transcription factor NF-kB (p50/p50, p65/p65) and FSH in control of porcine ovarian granulosa cells functions and the possible role of dimers p50/p50, p65/p65 in mediating FSH actions on these cells. Monolayer of primary granulosa cells was transfected with plasmids encoding human p50 cDNA and p65 cDNA, and cultured with or without addition of FSH (0, 1, 10 or 100 ng/ml). The accumulation of proteins p50 and p65...

The aim of our study was to elucidated the role of mTOR-dependent intracellular signalling pathway in control of ovarian functions. For this purpose, we have examine the effect of three mTOR inhibitors (resveratrol, curcumin and synthetic mTOR blocker MC 2183 – Mai et al. 20051, at the doses 0, 1, 10, 100 μg/ml) on apoptosis and steroidogenesis by cultured Japanese quail ovarian granulosa cells. The release of steroid hormones (progesterone and te...